Difference between revisions of "BLAST+ HowTo"
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= See also = | = See also = | ||
* [[HowTo_BLAST]] - How to use original(legacy) BLAST | * [[HowTo_BLAST]] - How to use original(legacy) BLAST | ||
| + | * [[http://www.ncbi.nlm.nih.gov/books/NBK1763/ BLAST command line online manual]] | ||
* http://blast.advbiocomp.com/ - AB-BLAST (a.k.a. wu-blast or BLAST-2.0). It claims better performance than NCBI BLAST, but you need to pay at least $320/yr if you want to use its output in your publication. | * http://blast.advbiocomp.com/ - AB-BLAST (a.k.a. wu-blast or BLAST-2.0). It claims better performance than NCBI BLAST, but you need to pay at least $320/yr if you want to use its output in your publication. | ||
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[[Category:HowTo]] | [[Category:HowTo]] | ||
Revision as of 20:56, 17 April 2014
Download & install the program
- ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/
- Old version: ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/
- If you download binary/executable files, executable files are available right after uncompress the file. Hereafter, '/path/to/ncbi+/' refer the directory of those files on your computer.
- If you need to compile the source code, read 'README' and/or 'INSTALL' document inside source directory after uncompress the file.
Make sequence database
For nucleotide sequence database,
$ /path/to/blast+/bin/makeblastdb -dbtype nucl <my_seq.fa> <my_db_name>
For protein sequence database,
$ /path/to/blast+/bin/makeblastdb -dbtype prot <my_seq.fa> <my_db_name>
Run sequence search
See also
- HowTo_BLAST - How to use original(legacy) BLAST
- [BLAST command line online manual]
- http://blast.advbiocomp.com/ - AB-BLAST (a.k.a. wu-blast or BLAST-2.0). It claims better performance than NCBI BLAST, but you need to pay at least $320/yr if you want to use its output in your publication.
